Enchancment of immunodetection of the transcription issue C/EBP homologous protein by westernblot.
Accumulation of misfolded proteins inside the endoplasmic reticulum (ER) induces an unfolded protein response (UPR) that both restores homeostasis or triggers apoptosis in case of adaptation failure. The three activated branches of UPR result in IRE1-, PERK- and ATF6– dependent transcriptional induction of the gene encoding the transcription issue C/EBP homologous protein (CHOP) which performs an vital function in apoptosis induction. In typical immunoblotting situations, detection of CHOP is a tough process. Utilizing a fixation step, now we have optimized the detection of CHOP and this technique supplies a worthwhile instrument to decipher CHOP involvement in UPR.
Toxicity of environmental ozone publicity on mice olfactory bulbs, utilizing Westernblot approach.
Environmental ozone (O3) publicity has opposed results on totally different physique programs. This experimental work aimed to review the impact(s) of O3 publicity on the olfactory bulbs (OB) of Swiss Webster and C57BL/6J mouse strains, utilizing Western blot approach. Each mice strains have been uncovered to totally different O3 doses for various variety of exposures and durations.
The outcomes indicated that O3 publicity precipitated a major enhance within the stage of the proteins concerned within the oxidative stress state corresponding to 4-hydroxynonenal (4HNE) and Cytochrome P450, household 1, subfamily A, polypeptide 1 (CYP1A1), along with the full OB proteins in Swiss Webster mouse pressure. Nonetheless, this impact was not noticed in C57BL/6J mouse pressure. Moreover, CYP1A1 was utterly absent within the Inexperienced fluorescent protein (GFP) C57BL/6J O3 uncovered mice. Furthermore, O3 publicity precipitated a major lower within the physique weight of the examined mice from the 2 strains.
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Mouse monocyte chemotactic protein 1/monocyte chemotactic and activating factor, MCP-1/MCAF ELISA kit
Description: Quantitativesandwich ELISA kit for measuring Mouse monocyte chemotactic protein 1/monocyte chemotactic and activating factor, MCP-1/MCAF in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Description: Quantitativesandwich ELISA kit for measuring Rabbit monocyte chemotactic protein 1/monocyte chemotactic and activating factor, MCP1/MCAF in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Monkey monocyte chemotactic protein 1/monocyte chemotactic and activating factor, MCP1/MCAF ELISA kit
Description: Quantitativesandwich ELISA kit for measuring Monkey monocyte chemotactic protein 1/monocyte chemotactic and activating factor, MCP1/MCAF in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Human Monocyte chemotactic protein 1/monocyte chemotactic and activating factor ELISA kit
Description: A competitive ELISA for quantitative measurement of Human Monocyte chemotactic protein 1/monocyte chemotactic and activating factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human Monocyte chemotactic protein 1/monocyte chemotactic and activating factor ELISA kit
Description: A competitive ELISA for quantitative measurement of Human Monocyte chemotactic protein 1/monocyte chemotactic and activating factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human Monocyte chemotactic protein 1/monocyte chemotactic and activating factor ELISA kit
Description: A competitive ELISA for quantitative measurement of Human Monocyte chemotactic protein 1/monocyte chemotactic and activating factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Goat Monocyte chemotactic protein 1/monocyte chemotactic and activating factor ELISA kit
Description: A competitive ELISA for quantitative measurement of Goat Monocyte chemotactic protein 1/monocyte chemotactic and activating factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Goat Monocyte chemotactic protein 1/monocyte chemotactic and activating factor ELISA kit
Description: A competitive ELISA for quantitative measurement of Goat Monocyte chemotactic protein 1/monocyte chemotactic and activating factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Goat Monocyte chemotactic protein 1/monocyte chemotactic and activating factor ELISA kit
Description: A competitive ELISA for quantitative measurement of Goat Monocyte chemotactic protein 1/monocyte chemotactic and activating factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Dog Monocyte chemotactic protein 1/monocyte chemotactic and activating factor ELISA kit
Description: A competitive ELISA for quantitative measurement of Canine Monocyte chemotactic protein 1/monocyte chemotactic and activating factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Dog Monocyte chemotactic protein 1/monocyte chemotactic and activating factor ELISA kit
Description: A competitive ELISA for quantitative measurement of Canine Monocyte chemotactic protein 1/monocyte chemotactic and activating factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Dog Monocyte chemotactic protein 1/monocyte chemotactic and activating factor ELISA kit
Description: A competitive ELISA for quantitative measurement of Canine Monocyte chemotactic protein 1/monocyte chemotactic and activating factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rat Monocyte chemotactic protein 1/monocyte chemotactic and activating factor ELISA kit
Description: A competitive ELISA for quantitative measurement of Rat Monocyte chemotactic protein 1/monocyte chemotactic and activating factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rat Monocyte chemotactic protein 1/monocyte chemotactic and activating factor ELISA kit
Description: A competitive ELISA for quantitative measurement of Rat Monocyte chemotactic protein 1/monocyte chemotactic and activating factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rat Monocyte chemotactic protein 1/monocyte chemotactic and activating factor ELISA kit
Description: A competitive ELISA for quantitative measurement of Rat Monocyte chemotactic protein 1/monocyte chemotactic and activating factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Mouse Monocyte chemotactic protein 1/monocyte chemotactic and activating factor ELISA kit
Description: A competitive ELISA for quantitative measurement of Mouse Monocyte chemotactic protein 1/monocyte chemotactic and activating factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Mouse Monocyte chemotactic protein 1/monocyte chemotactic and activating factor ELISA kit
Description: A competitive ELISA for quantitative measurement of Mouse Monocyte chemotactic protein 1/monocyte chemotactic and activating factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Mouse Monocyte chemotactic protein 1/monocyte chemotactic and activating factor ELISA kit
Description: A competitive ELISA for quantitative measurement of Mouse Monocyte chemotactic protein 1/monocyte chemotactic and activating factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rabbit Monocyte chemotactic protein 1/monocyte chemotactic and activating factor ELISA kit
Description: A competitive ELISA for quantitative measurement of Rabbit Monocyte chemotactic protein 1/monocyte chemotactic and activating factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rabbit Monocyte chemotactic protein 1/monocyte chemotactic and activating factor ELISA kit
Description: A competitive ELISA for quantitative measurement of Rabbit Monocyte chemotactic protein 1/monocyte chemotactic and activating factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rabbit Monocyte chemotactic protein 1/monocyte chemotactic and activating factor ELISA kit
Description: A competitive ELISA for quantitative measurement of Rabbit Monocyte chemotactic protein 1/monocyte chemotactic and activating factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Pig Monocyte chemotactic protein 1/monocyte chemotactic and activating factor ELISA kit
Description: A competitive ELISA for quantitative measurement of Porcine Monocyte chemotactic protein 1/monocyte chemotactic and activating factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Pig Monocyte chemotactic protein 1/monocyte chemotactic and activating factor ELISA kit
Description: A competitive ELISA for quantitative measurement of Porcine Monocyte chemotactic protein 1/monocyte chemotactic and activating factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Pig Monocyte chemotactic protein 1/monocyte chemotactic and activating factor ELISA kit
Description: A competitive ELISA for quantitative measurement of Porcine Monocyte chemotactic protein 1/monocyte chemotactic and activating factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Monkey Monocyte chemotactic protein 1/monocyte chemotactic and activating factor ELISA kit
Description: A competitive ELISA for quantitative measurement of Monkey Monocyte chemotactic protein 1/monocyte chemotactic and activating factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Monkey Monocyte chemotactic protein 1/monocyte chemotactic and activating factor ELISA kit
Description: A competitive ELISA for quantitative measurement of Monkey Monocyte chemotactic protein 1/monocyte chemotactic and activating factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Monkey Monocyte chemotactic protein 1/monocyte chemotactic and activating factor ELISA kit
Description: A competitive ELISA for quantitative measurement of Monkey Monocyte chemotactic protein 1/monocyte chemotactic and activating factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Bovine monocyte chemotactic protein 1/monocyte chemotactic and activating factor, (MCP-1/CCL2/MCAF)ELISA Kit
Description: Quantitativesandwich ELISA kit for measuring Bovine monocyte chemotactic protein 1/monocyte chemotactic and activating factor, (MCP-1/CCL2/MCAF) in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Description: Human MCP-1, generated by the proteolytic removal of the signal peptide and propeptide and has a calculated molecular mass of approximately 9 kDa. Recombinant MCP-1 is a disulfide-linked monomeric protein consisting of 77 amino acid residues and migrates as an approximately 9 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding human MCP-1 (CCL2) mature chain was expressed in E. coli.
Description: Human MCP-1, generated by the proteolytic removal of the signal peptide and propeptide and has a calculated molecular mass of approximately 9 kDa. Recombinant MCP-1 is a disulfide-linked monomeric protein consisting of 77 amino acid residues and migrates as an approximately 9 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding human MCP-1 (CCL2) mature chain was expressed in E. coli.
MCP-4 Monocyte Chemotactic Protein-4 Human Recombinant Protein (CCL13)
Description: Monocyte Chemotactic Protein-4 Human Recombinant produced in E.Coli is a non-glycosylated, Polypeptide chain containing 75 amino acids and having a molecular mass of 8.6 kDa. The MCP-4 is purified by proprietary chromatographic techniques.
Description: A competitive ELISA for quantitative measurement of Guinea pig Monocyte chemotactic protein 1/monocyte chemotactic and activating factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Guinea pig Monocyte chemotactic protein 1/monocyte chemotactic and activating factor ELISA kit
Description: A competitive ELISA for quantitative measurement of Guinea pig Monocyte chemotactic protein 1/monocyte chemotactic and activating factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Guinea pig Monocyte chemotactic protein 1/monocyte chemotactic and activating factor ELISA kit
Description: A competitive ELISA for quantitative measurement of Guinea pig Monocyte chemotactic protein 1/monocyte chemotactic and activating factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Pig monocyte chemotactic protein 1, MCP1 ELISA Kit
Description: Quantitativesandwich ELISA kit for measuring Pig monocyte chemotactic protein 1, MCP1 in samples from serum, plasma. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Human Monocyte Chemotactic Protein 1 (MCP1) Protein
Description: Quantitative sandwich ELISA kit for measuring Human monocyte chemotactic protein 1/monocyte chemotactic and activating factor, MCP-1/MCAF in samples from serum, plasma, urine, saliva, cerebrospinalfluid (CSF), asciticfluid, cell culture supernates, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Dog Monocyte Chemotactic Protein 1/Monocyte Chemotactic And Activating Factor(MCP-1/MCAF) ELISA kit
Description: Quantitative sandwich ELISA kit for measuring Dog Monocyte Chemotactic Protein 1/Monocyte Chemotactic And Activating Factor (MCP-1/MCAF) in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Rat monocyte chemotactic protein 1/monocyte chemotactic and activating factor, MCP-1/MCAF ELISA kit
Description: Quantitativesandwich ELISA kit for measuring Rat monocyte chemotactic protein 1/monocyte chemotactic and activating factor, MCP-1/MCAF in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Mouse monocyte chemotactic protein 1/monocyte chemotactic and activating factor, MCP-1/MCAF ELISA kit
Description: Quantitativesandwich ELISA kit for measuring Mouse monocyte chemotactic protein 1/monocyte chemotactic and activating factor, MCP-1/MCAF in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Human monocyte chemotactic protein 1/monocyte chemotactic and activating factor,MCP-1/MCAF ELISA kit
Description: Quantitativesandwich ELISA kit for measuring Rabbit monocyte chemotactic protein 1/monocyte chemotactic and activating factor, MCP1/MCAF in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Monkey monocyte chemotactic protein 1/monocyte chemotactic and activating factor, MCP1/MCAF ELISA kit
Description: Quantitativesandwich ELISA kit for measuring Monkey monocyte chemotactic protein 1/monocyte chemotactic and activating factor, MCP1/MCAF in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Monkey monocyte chemotactic protein 1/monocyte chemotactic and activating factor,MCP1/MCAF ELISA kit
Examine of penetration mechanism of labrasol on rabbit cornea by Ussing chamber, RT-PCR assay, Westernblot and immunohistochemistry.
Labrasol, as a non-ionic surfactant, can improve the permeation and absorption of medicine, and is extensively utilized in topical, transdermal, and oral pharmaceutical preparations as an emulsifier and absorption enhancer. Latest research in our laboratory have indicated that labrasol has a powerful absorption enhancing impact on several types of medicine in vitro and in vivo. This examine was carried out to additional elucidate the motion mechanism of labrasol on the corneal penetration.
On this analysis, the fluorescein sodium, a marker of passive paracellular transport of tight junction, was chosen because the mannequin drug to evaluate the impact of labrasol on in vitro corneal permeability.
To research the continual and real-time affect of labrasol on the membrane permeability and integrity, the Ussing chamber system was utilized to observe the electrophysiological parameters. And, moreover, we elucidated the impact of labrasol on excised cornea on the molecular stage by software of RT-PCR, Western blot, and immunohistochemical staining.
The outcomes indicated that labrasol clearly improve the transcorneal permeability of fluorescein sodium, and the enhancement was realized by interacting with and down-regulating the related proteins, corresponding to F-actin, claudin-1 and β-catenin, which have been contributed to cell-cell connections, respectively.
Optimization of blocking situations for fluorescent Westernblot.
Blocking situations are crucial for lowering background and non-specific alerts in Western blot (WB). For fluorescent WB, nonetheless, blockers might herald extra autofluorescence, and optimum blocking situations have been much less nicely established. Right here, the autofluorescence of Tween 20 is clarified, which is negligible when imaged moist, however could possibly be vital when Tween 20 is dried on blots. In most conditions, blocking with Tween 20 reduces background and/or improves particular alerts. Systematical investigation of blocking situations for fluorescent WB reveals that the autofluorescent background is set by a mix of a number of components together with the blocker, the excitation/emission configuration, the membrane, and the H2O (moist or dry), various case by case.
The generally used protein or polymer blockers (milk, bovine serum albumin, fish gelatin, and polyvinylpyrrolidone) every have distinctive benefits and drawbacks in regards to the autofluorescence, the blocking effectivity, and the associated fee. 0.005%-0.02% sodium dodecyl sulphate will be included to scale back non-specific bands and background attributable to non-specific binding of antibodies, nonetheless, at the price of additionally impairing particular alerts. For poorly-performing antibodies, a pre-test could be essential to determine an acceptable blocker. This work supplies a reference for cost-effective blocking situations for fluorescent WB.
Growth of an ADP-ribosylation assay for residual toxicity in C. difficile binary toxin CDTa utilizing automated capillary westernblot.
CDTa, an actin ADP-ribosylation transferase, is a binary toxin produced by the bacterium Clostridium difficile which is often related to the hypervirulent pressure current in Clostridium difficile infections. The mutated type of CDTa, 4mCDTa, is among the parts within the tetravalent Clostridium difficile vaccine wherein the residual toxicity of the ADP-ribosylation exercise must be monitored for security causes.
There are a number of ADP- ribosylation exercise strategies using methods corresponding to ELISA, guide Western blot, or SDS PAGE, however all these strategies are often time consuming and labor intensive. Right here we describe the event of latest quantitative capillary primarily based western for monitoring the presence of ADP-ribosylation exercise in CDTa and 4mCDTa utilizing novel, automated Easy Western™ expertise. Moreover, now we have measured for the primary time the enzyme’s kinetic parameters, OkayM (NAD) and okaycat for native CDTa utilizing this new quantitative capillary western expertise.
Description: A monoclonal antibody for detection of GAPDH from Human, Mouse, Rat, Monkey, Dog, Chicken, Rabbit, Pig, Sheep, Insect, Yeast, Hamster. This GAPDH antibody is for WB, IHC-P, IF. It is affinity-purified from mouse ascites by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in mouse by using as an immunogen synthetic peptide
Description: A monoclonal antibody for detection of GAPDH from Human, Mouse, Rat, Monkey, Dog, Chicken, Rabbit, Pig, Sheep, Insect, Yeast, Hamster. This GAPDH antibody is for WB, IHC-P, IF. It is affinity-purified from mouse ascites by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in mouse by using as an immunogen synthetic peptide
Description: A monoclonal antibody for detection of GAPDH from Human, Mouse, Rat, Monkey, Dog, Chicken, Rabbit, Pig, Sheep, Insect, Yeast, Hamster. This GAPDH antibody is for WB, IHC-P, IF. It is affinity-purified from mouse ascites by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in mouse by using as an immunogen synthetic peptide
Description: A monoclonal antibody for detection of GAPDH from Human, Mouse, Rat, Monkey, Dog, Chicken, Rabbit, Pig, Sheep, Insect, Yeast, Hamster. This GAPDH antibody is for IHC-P, IF. It is affinity-purified from mouse ascites by affinity-chromatography using the specific immunogen and conjugated to AbFluor? 350. The antibody is produced in mouse by using as an immunogen synthetic peptide
Description: A monoclonal antibody for detection of GAPDH from Human, Mouse, Rat, Monkey, Dog, Chicken, Rabbit, Pig, Sheep, Insect, Yeast, Hamster. This GAPDH antibody is for IHC-P, IF. It is affinity-purified from mouse ascites by affinity-chromatography using the specific immunogen and conjugated to AbFluor? 405. The antibody is produced in mouse by using as an immunogen synthetic peptide
Description: A monoclonal antibody for detection of GAPDH from Human, Mouse, Rat, Monkey, Dog, Chicken, Rabbit, Pig, Sheep, Insect, Yeast, Hamster. This GAPDH antibody is for IHC-P, IF. It is affinity-purified from mouse ascites by affinity-chromatography using the specific immunogen and conjugated to AbFluor? 488. The antibody is produced in mouse by using as an immunogen synthetic peptide
Description: A monoclonal antibody for detection of GAPDH from Human, Mouse, Rat, Monkey, Dog, Chicken, Rabbit, Pig, Sheep, Insect, Yeast, Hamster. This GAPDH antibody is for IHC-P, IF. It is affinity-purified from mouse ascites by affinity-chromatography using the specific immunogen and conjugated to AbFluor? 555. The antibody is produced in mouse by using as an immunogen synthetic peptide
Description: A monoclonal antibody for detection of GAPDH from Human, Mouse, Rat, Monkey, Dog, Chicken, Rabbit, Pig, Sheep, Insect, Yeast, Hamster. This GAPDH antibody is for IHC-P, IF. It is affinity-purified from mouse ascites by affinity-chromatography using the specific immunogen and conjugated to AbFluor? 594. The antibody is produced in mouse by using as an immunogen synthetic peptide