December 1, 2023

Evaluation in animal fashions of myocardial ischemic infarction

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An acceptable loading management for western blot evaluation in animal fashions of myocardial ischemic infarction.

An acceptable loading management is crucial for Western blot evaluation. Housekeeping proteins (HKPs), corresponding to β-actin, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and β-tubulin, are generally used to normalize protein expression.

However HKP expression will be impacted by sure experimental situations, corresponding to ischemic myocardial infarction. This examine was undertaken to search for an acceptable loading management for western blot evaluation of ischemic myocardium.

Myocardial ischemic infarction was induced by left anterior descending coronary artery (LAD) ligation in Rhesus monkeys and C57BL/6 mice. The guts tissue samples from totally different areas and time factors after surgical procedure have been subjected to western blot or gel staining.

The extent of β-actin, GAPDH, β-tubulin, and whole protein have been examined. The full protein stage was constant in all teams, whereas the protein stage of β-tubulin and β-actin have been totally different in all teams.

Nonetheless, the protein stage of GAPDH was secure within the Rhesus monkey mannequin. We concluded that whole protein was probably the most acceptable inside management in numerous phases of myocardial ischemic illness of assorted animal fashions. GAPDH is a dependable inside management just for ischemic myocardium of Rhesus monkey.

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EED Western Blot kit (AWBK38384)

AWBK38384 10 reactions
EUR 776.4
Description:
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SSB Western Blot kit (AWBK40462)

AWBK40462 10 reactions
EUR 776.4
Description:
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OTC Western Blot kit (AWBK41766)

AWBK41766 10 reactions
EUR 776.4
Description:
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FST Western Blot kit (AWBK42287)

AWBK42287 10 reactions
EUR 776.4
Description:
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TMB Substrate, for Western Blot

F100029 1000 ml
EUR 992.4
Description: TMB Substrate, for Western Blot by Cygnus Technologies is available in Europe via Gentaur.

TMB Substrate, for Western Blot

F129-100 100 ml
EUR 298.8
Description: TMB Substrate, for Western Blot by Cygnus Technologies is available in Europe via Gentaur.

ERG Western Blot kit (AWBK100625)

AWBK100625 10 reactions
EUR 776.4
Description:
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GLI2 Western Blot kit (AWBK31885)

AWBK31885 10 reactions
EUR 776.4
Description:
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PAX4 Western Blot kit (AWBK32064)

AWBK32064 10 reactions
EUR 776.4
Description:
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GLI1 Western Blot kit (AWBK32368)

AWBK32368 10 reactions
EUR 776.4
Description:
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PAX7 Western Blot kit (AWBK32393)

AWBK32393 10 reactions
EUR 776.4
Description:
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LHX6 Western Blot kit (AWBK32553)

AWBK32553 10 reactions
EUR 776.4
Description:
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PAX7 Western Blot kit (AWBK32742)

AWBK32742 10 reactions
EUR 776.4
Description:
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SOX5 Western Blot kit (AWBK33323)

AWBK33323 10 reactions
EUR 776.4
Description:
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A1BG Western Blot kit (AWBK33810)

AWBK33810 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity: Human
  • Application:
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NPM1 Western Blot kit (AWBK34094)

AWBK34094 10 reactions
EUR 776.4
Description:
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LIG4 Western Blot kit (AWBK34122)

AWBK34122 10 reactions
EUR 776.4
Description:
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RNF2 Western Blot kit (AWBK34290)

AWBK34290 10 reactions
EUR 776.4
Description:
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  • Species reactivity:
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ILF2 Western Blot kit (AWBK35731)

AWBK35731 10 reactions
EUR 776.4
Description:
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  • Species reactivity:
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SPP1 Western Blot kit (AWBK36677)

AWBK36677 10 reactions
EUR 776.4
Description:
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ZBP1 Western Blot kit (AWBK36711)

AWBK36711 10 reactions
EUR 776.4
Description:
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G3BP Western Blot kit (AWBK37713)

AWBK37713 10 reactions
EUR 776.4
Description:
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BRD7 Western Blot kit (AWBK39018)

AWBK39018 10 reactions
EUR 776.4
Description:
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MSI2 Western Blot kit (AWBK41112)

AWBK41112 10 reactions
EUR 776.4
Description:
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  • Species reactivity:
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TPM1 Western Blot kit (AWBK41392)

AWBK41392 10 reactions
EUR 776.4
Description:
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  • Species reactivity:
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TSHR Western Blot kit (AWBK41870)

AWBK41870 10 reactions
EUR 776.4
Description:
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  • Species reactivity:
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ASPN Western Blot kit (AWBK42487)

AWBK42487 10 reactions
EUR 776.4
Description:
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  • Species reactivity:
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RORA Western Blot kit (AWBK45608)

AWBK45608 10 reactions
EUR 776.4
Description:
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  • Species reactivity:
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PDCD4 Western Blot kit (AWBK09045)

AWBK09045 10 reactions
EUR 776.4
Description:
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ETS1 Western Blot kit (AWBK100604)

AWBK100604 10 reactions
EUR 776.4
Description:
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  • Species reactivity:
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GABRP Western Blot kit (AWBK13034)

AWBK13034 10 reactions
EUR 776.4
Description:
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  • Species reactivity:
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HTR1A Western Blot kit (AWBK13041)

AWBK13041 10 reactions
EUR 776.4
Description:
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  • Species reactivity:
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RAB14 Western Blot kit (AWBK13107)

AWBK13107 10 reactions
EUR 776.4
Description:
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  • Species reactivity:
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TNRC4 Western Blot kit (AWBK31525)

AWBK31525 10 reactions
EUR 776.4
Description:
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  • Species reactivity:
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ESRRG Western Blot kit (AWBK31655)

AWBK31655 10 reactions
EUR 776.4
Description:
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  • Species reactivity:
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HIPK2 Western Blot kit (AWBK32586)

AWBK32586 10 reactions
EUR 776.4
Description:
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  • Species reactivity:
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ZFP67 Western Blot kit (AWBK32749)

AWBK32749 10 reactions
EUR 776.4
Description:
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OLIG2 Western Blot kit (AWBK32753)

AWBK32753 10 reactions
EUR 776.4
Description:
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  • Species reactivity:
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SOX10 Western Blot kit (AWBK33326)

AWBK33326 10 reactions
EUR 776.4
Description:
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RPS16 Western Blot kit (AWBK33535)

AWBK33535 10 reactions
EUR 776.4
Description:
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  • Species reactivity:
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ATP7A Western Blot kit (AWBK33797)

AWBK33797 10 reactions
EUR 776.4
Description:
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  • Species reactivity:
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ZFP36 Western Blot kit (AWBK34385)

AWBK34385 10 reactions
EUR 776.4
Description:
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  • Species reactivity:
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ARIH2 Western Blot kit (AWBK34418)

AWBK34418 10 reactions
EUR 776.4
Description:
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  • Species reactivity:
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KEAP1 Western Blot kit (AWBK34728)

AWBK34728 10 reactions
EUR 776.4
Description:
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  • Species reactivity:
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CLIC1 Western Blot kit (AWBK35045)

AWBK35045 10 reactions
EUR 776.4
Description:
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KCNN2 Western Blot kit (AWBK35094)

AWBK35094 10 reactions
EUR 776.4
Description:
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  • Species reactivity:
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CLCN3 Western Blot kit (AWBK35504)

AWBK35504 10 reactions
EUR 776.4
Description:
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P2RX7 Western Blot kit (AWBK35518)

AWBK35518 10 reactions
EUR 776.4
Description:
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  • Species reactivity:
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TRPM3 Western Blot kit (AWBK35609)

AWBK35609 10 reactions
EUR 776.4
Description:
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  • Species reactivity:
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PITX2 Western Blot kit (AWBK35634)

AWBK35634 10 reactions
EUR 776.4
Description:
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  • Species reactivity:
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ZFP42 Western Blot kit (AWBK36244)

AWBK36244 10 reactions
EUR 776.4
Description:
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  • Species reactivity:
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ANXA1 Western Blot kit (AWBK36568)

AWBK36568 10 reactions
EUR 776.4
Description:
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  • Species reactivity:
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RUNX2 Western Blot kit (AWBK36678)

AWBK36678 10 reactions
EUR 776.4
Description:
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  • Species reactivity:
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Bapx1 Western Blot kit (AWBK36826)

AWBK36826 10 reactions
EUR 776.4
Description:
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  • Species reactivity:
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SATB1 Western Blot kit (AWBK38208)

AWBK38208 10 reactions
EUR 776.4
Description:
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  • Species reactivity:
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JMJD3 Western Blot kit (AWBK40102)

AWBK40102 10 reactions
EUR 776.4
Description:
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  • Species reactivity:
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STAU1 Western Blot kit (AWBK40537)

AWBK40537 10 reactions
EUR 776.4
Description:
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  • Species reactivity:
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PCBP1 Western Blot kit (AWBK40630)

AWBK40630 10 reactions
EUR 776.4
Description:
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  • Species reactivity:
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WNT2B Western Blot kit (AWBK41254)

AWBK41254 10 reactions
EUR 776.4
Description:
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  • Species reactivity:
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GNB1L Western Blot kit (AWBK41328)

AWBK41328 10 reactions
EUR 776.4
Description:
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  • Species reactivity:
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STK11 Western Blot kit (AWBK41743)

AWBK41743 10 reactions
EUR 776.4
Description:
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  • Species reactivity:
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KRT13 Western Blot kit (AWBK42031)

AWBK42031 10 reactions
EUR 776.4
Description:
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  • Species reactivity:
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IDH3A Western Blot kit (AWBK42237)

AWBK42237 10 reactions
EUR 776.4
Description:
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  • Species reactivity:
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PBEF1 Western Blot kit (AWBK42255)

AWBK42255 10 reactions
EUR 776.4
Description:
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  • Species reactivity:
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RSAD2 Western Blot kit (AWBK42696)

AWBK42696 10 reactions
EUR 776.4
Description:
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  • Species reactivity:
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  • Assay info:
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PANX2 Western Blot kit (AWBK42778)

AWBK42778 10 reactions
EUR 776.4
Description:
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  • Species reactivity:
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PANX1 Western Blot kit (AWBK42783)

AWBK42783 10 reactions
EUR 776.4
Description:
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  • Species reactivity:
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TGFBI Western Blot kit (AWBK44268)

AWBK44268 10 reactions
EUR 776.4
Description:
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  • Species reactivity:
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PTPN2 Western Blot kit (AWBK45362)

AWBK45362 10 reactions
EUR 776.4
Description:
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  • Species reactivity:
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TWIST1 Western Blot kit (AWBK37997)

AWBK37997 10 reactions
EUR 776.4
Description:
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  • Species reactivity:
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TARDBP Western Blot kit (AWBK38941)

AWBK38941 10 reactions
EUR 776.4
Description:
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  • Species reactivity:
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TARDBP Western Blot kit (AWBK38942)

AWBK38942 10 reactions
EUR 776.4
Description:
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  • Species reactivity:
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SNRP70 Western Blot kit (AWBK40439)

AWBK40439 10 reactions
EUR 776.4
Description:
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  • Species reactivity:
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HNRPLL Western Blot kit (AWBK41102)

AWBK41102 10 reactions
EUR 776.4
Description:
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  • Species reactivity:
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HNRPA3 Western Blot kit (AWBK41195)

AWBK41195 10 reactions
EUR 776.4
Description:
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  • Species reactivity:
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  • Assay info:
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SLC1A5 Western Blot kit (AWBK42247)

AWBK42247 10 reactions
EUR 776.4
Description:
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  • Species reactivity:
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ONECUT2 Western Blot kit (AWBK35750)

AWBK35750 10 reactions
EUR 776.4
Description:
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  • Species reactivity:
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SLC12A1 Western Blot kit (AWBK41388)

AWBK41388 10 reactions
EUR 776.4
Description:
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  • Species reactivity:
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CEACAM6 Western Blot kit (AWBK41504)

AWBK41504 10 reactions
EUR 776.4
Description:
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  • Species reactivity:
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SLC22A1 Western Blot kit (AWBK41516)

AWBK41516 10 reactions
EUR 776.4
Description:
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  • Species reactivity:
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RHOBTB1 Western Blot kit (AWBK41883)

AWBK41883 10 reactions
EUR 776.4
Description:
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  • Species reactivity:
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  • Assay info:
  • Sensitivity:

SLC22A7 Western Blot kit (AWBK42306)

AWBK42306 10 reactions
EUR 776.4
Description:
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  • Species reactivity:
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  • Assay info:
  • Sensitivity:

Interferon alpha Western Blot Antibody

20-abx137014
  • EUR 427.20
  • EUR 627.60
  • 0.5 mg
  • 1 mg

Colorimetric Western Blot Kit (OKRA00014)

OKRA00014 1 kit
EUR 730.8
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

Colorimetric Western Blot Kit (OKRA00015)

OKRA00015 1 each
EUR 730.8
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

Colorimetric Western Blot Kit (OKRA00016)

OKRA00016 1 kit
EUR 730.8
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

Colorimetric Western Blot Kit (OKRA00017)

OKRA00017 1 kit
EUR 730.8
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

cyclin G2 Western Blot kit (AWBK03032)

AWBK03032 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

Pure-IP Western Blot Detection Kit

PRB-5002 20 blots
EUR 470.4
Description: Western blot detection of IP proteins can exhibit high background due to the use of the same primary antibody in the IP and the Western blot. The Pure-IP Western Blot Detection Kit eliminates this background problem by using a proprietary HRP Conjugate for detection of the primary antibody.

Chemiluminescent Western Blot Kit (OKRA00001)

OKRA00001 1 kit
EUR 684
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

Chemiluminescent Western Blot Kit (OKRA00002)

OKRA00002 1 kit
EUR 684
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

Chemiluminescent Western Blot Kit (OKRA00003)

OKRA00003 1 kit
EUR 684
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

Chemiluminescent Western Blot Kit (OKRA00004)

OKRA00004 1 kit
EUR 684
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

T-Pro Western Blot Stripping Reagent

JB11-K002 500ml/BT
EUR 213.6

AgileBlotter? Automated Western Blot Processor

ACT-ABH-40 each
EUR 20326.8

Nitrated Mol Wt Std for Western blot

NITT12-C 100 ul
EUR 343.2

West-Ez Blocking Buffer, 1 % Casein for Western Blot

W3720-050 500ml
EUR 144

West-Ez Blocking Buffer, 1 % Casein for Western Blot

W3720-100 2x500ml
EUR 176.4

Bovine DNAse 1 Protein Western blot +ve control

DNASE11-C 100 ul
EUR 343.2

Mouse Leptin protein Western blot +ve control # 1

LEP11-C 100 ul
EUR 343.2

Western Blot Primary Antibody Diluent Buffer

abx090689-100ml 100 ml
EUR 218.4

Attoglow Western Blot Analysis Kit:Blocking Agent

K3171250-3 40 g
EUR 68

Hypersensitive Western Blot Chemiluminescent Substrate

CR0001-10 20mL (sufficient reagents for 4000 cm2 of membrane)
EUR 200.4

Hypersensitive Western Blot Chemiluminescent Substrate

CR0001-25 50mL (sufficient reagents for 10000 cm2 of membrane)
EUR 376.8

Hypersensitive Western Blot Chemiluminescent Substrate

CR0001-5 10mL (sufficient reagents for 2000 cm2 of membrane)
EUR 139.2

Recombinant Human MutY Protein for Western blot

MUTY11-C 100 ul
EUR 343.2

Human Liver Cathepsin B protein control for Western blot

CTHB11-C 100 ul
EUR 343.2

Human Liver Cathepsin D protein control for Western blot

CTHD11-C 100 ul
EUR 343.2

Human Liver Cathepsin G protein control for Western blot

CTHG11-C 100 ul
EUR 343.2

Human Liver Cathepsin H protein control for Western blot

CTHH11-C 100 ul
EUR 343.2

Human Liver Cathepsin L protein control for Western blot

CTHL11-C 100 ul
EUR 343.2

ChonBlock Western Blot Buffer 2 for Goat (PBS)

90691P 10 ml
EUR 145.26
Description: ChonBlock Western Blot Buffer 2 for Goat (PBS)

ChonBlock Western Blot Buffer 2 for Goat (TBS)

90691T 10 ml
EUR 145.26
Description: ChonBlock Western Blot Buffer 2 for Goat (TBS)

Poly-ADP-ribosylated proteins for Western blot

ADPR11-C 100 ul
EUR 343.2

Rat Ceruloplasmin protein control for western blot

CP17-C 100 ul
EUR 343.2

Attoglow Western Blot Analysis Kit:Binding Buffer 20x

K3171250-2 120 ml
EUR 107

SuperBrite? ECL Western Blot Substrate/Detection Kit

K824-50 each
EUR 229.2

ChonBlock Western Blot Buffer 2 for Rabbit (PBS)

90692P 10 ml
EUR 145.26
Description: ChonBlock Western Blot Buffer 2 for Rabbit (PBS)

ChonBlock Western Blot Buffer 2 for Rabbit (TBS)

90692T 10 ml
EUR 145.26
Description: ChonBlock Western Blot Buffer 2 for Rabbit (TBS)

Human Leptin protein Western blot +ve control # 2

LEP12-C 100 ul
EUR 343.2

Mouse Ceruloplasmin protein control for western blot

CP16-C 100 ul
EUR 343.2

Purified Ram Cyclooxygenase (Cox-1) Western blot +ve control

COX11-C 100 ul
EUR 343.2

Western Blot Box - 6 x 4 x 1 1/4in.; 15.2 x 10.2 x 3.2cm

B1200-17 5/pack
EUR 107.63
Description: Western Blot Boxes

Western blot recycling stripping buffer, 50 ml (10X)

90101 50 ml
EUR 285.6

Box Western Blot Polystyrene Transparent 95x30x16mm X5

DD62676 PK5
EUR 99.6

Attoglow Western Blot Analysis Kit: Millennium Enhancer

K3171250-1 100 ml
EUR 130

Purified Human C1q protein Western blot +ve control

C1Q11-C 100 ul
EUR 343.2

HRP Western Blot Anti-IgG Antibody Kit (OKRA00006)

OKRA00006 100 ug
EUR 246
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

Human C1 Esterase Inhibitor western blot +ve control

C1E11-C 100 ul
EUR 343.2

WesternOne? Marker Pen, HRP for Western blot chemiluminescent detection

ACT-WP each
EUR 172.8

Human Liver Ferritin protein Western blot +ve control

FERT12-C 100 ul
EUR 343.2

NuGlo (luminol) ECL Substrate Kit for Western blot, 250 mls

80215-250 1 kit
EUR 196.8

Mouse NOS-II/i-NOS Protein Western Blot +ve Control # 1

iNOS-C 100 ul
EUR 343.2

Beta-Galactosidase (E. Coli) Protein for Western Blot

BGAL11-C 100 ul
EUR 343.2

Human Spleen Ferritin protein Western blot +ve control

FERT11-C 100 ul
EUR 343.2

Horse Spleen Ferritin protein Western blot +ve control

FERT13-C 100 ul
EUR 343.2

Chicken Serum Albumin protein control for Western blot

ALBC15-C 100 ul
EUR 343.2

Purified Cholera Toxin protein control for Western Blot

CTOX11-C 100 ul
EUR 343.2

Western Blot Box - 2 7/8 x 2 x 1 1/4in.; 7.3 x 5.1 x 3.2cm

B1200-5 5/pack
EUR 81.38
Description: Western Blot Boxes

Human Pancreatic Amylase protein control for Western blot

PAMY13-C 100 ul
EUR 343.2

Western Blot Box - 6 x 4 x 2in.; 15.2 x 10.2 x 5.1cm

B1200-19 5/pack
EUR 108.6
Description: Western Blot Boxes

Camelpox virus H3L/p35 protein control for western blot

CPOX11-C 100 ul
EUR 343.2

Human Adipsin/Factor D protein Western blot +ve control

ADN12-C 100 ul
EUR 343.2

Zika Virus prM Protein (African) control for Western blot

ZPRM11-C 100 ul
EUR 343.2

Recombinant Beta Lactamase protein control for Western blot

BLAC11-C 100 ul
EUR 343.2

Western Blot Box - 4 5/8 x 3 1/2 x 1 1/8in.; 11.7 x 8.9 x 2.8cm

B1200-13 5/pack
EUR 96.94
Description: Western Blot Boxes

Western Blot Box - 4 5/8 x 3 1/2 x 1 1/8in.; 11.7 x 8.9 x 2.8cm

B1200-13BK 5/pack
EUR 97.91
Description: Western Blot Boxes

Western Blot Box - 4 3/8 x 2 1/16 x 1 7/8in.; 11.1x 5.2 x 4.7cm

B1200-11 5/pack
EUR 94.02
Description: Western Blot Boxes

HRP Western Blot Anti-Rabbit IgG Antibody Kit (OKRA00007)

OKRA00007 100 ug
EUR 246
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

NuGlo (luminol) ECL Substrate Kit for Western blot, 50 mls

80215-250-S 1 kit
EUR 169.2

Western Blot Box - 3 1/2 x 2 9/16 x 1in.; 8.9 x 6.5 x 2.5cm

B1200-7 5/pack
EUR 88.19
Description: Western Blot Boxes

Western Blot Box - 3 1/2 x 2 9/16 x 1in.; 8.9 x 6.5 x 2.5cm

B1200-7BK 5/pack
EUR 90.13
Description: Western Blot Boxes

Recombinant human BAFF-R protein control for Western blot

BAFFR11-C 100 ul
EUR 343.2

Purified rat serum albumin protein control for Western blot

ALBR12-C 100 ul
EUR 343.2

Analysis of Western blot, ELISA and latex agglutination assessments to detect Toxoplasma gondii serum antibodies in farmed purple deer.

Abortion as a result of Toxoplasma gondii has been suspected in New Zealand farmed purple deer. Nonetheless, data across the epidemiology and prevalence of T. gondii in farmed purple deer is proscribed. The intention of this examine was to firstly, assess the sensitivity and specificity of two commercially accessible assays, ELISA and latex agglutination take a look at (LAT), to be used in deer and secondly, to estimate the sero-prevalence of T. gondii in purple deer. A complete of 252 sera from rising 2-year-old and grownup hinds from 17 New Zealand purple deer herds at early and late being pregnant scanning and from recognized aborted and/or non-aborted hinds have been examined for the presence of T. gondii antibodies.

Every assays’ sensitivity and specificity was evaluated by each the Western Blot (WB) as a gold commonplace technique and Bayesian latent class (BLC) evaluation within the absence of a gold commonplace.

The sensitivity and specificity for WB have been 95.8% (95% credible interval: 89.5-99.2%) and 95.1% (95% credible interval: 90.6-98.1%), respectively. For the LAT on the producer’s advisable ≥1:32 cut-off titre, the sensitivity (88.7%, 95% credible interval: 80.8-94.7%) and specificity (74.3%, 95% credible interval: 67.5-80.5%) have been decrease and better than the sensitivity (76.2%, 95% credible interval: 66.7-84.5%) and specificity (89.7%, 95% credible interval: 84.5-93.9%) at a ≥1:64 cut-off, utilizing (BLC) evaluation.

Sensitivity and specificity of the LAT at cut-off titre of 1:32 have been estimated to be 84.4% (95% CI: 74.9-90.9%) and 73.5% (95% CI: 65.8-79.9%) towards WB. The LAT had higher settlement with WB at cut-off titre of ≥1:64 than ≥1:32 (Kappa=0.63 vs 0.54). At optimised cut-off S/P of 15.5%, the sensitivity (98.8%, 95% credible interval 96.1-99.8%) and specificity (92.8%, 95% credible interval 88.9-95.7%) of the ELISA have been greater and decrease, respectively, than the sensitivity (85.1%, 95% credible interval 76.2-91.9%) and specificity (98.5%, 95% credible interval 96.9-99.4%) at producer’s cut-off S/P of 30%, from BLC evaluation. The sensitivity and specificity of ELISA at S/P cut-off of 15.5% was 91.1% (95% CI: 83.2-96.1%) and 90.7% (95% CI: 85.2-94.7%), respectively, when assessed towards WB. The sero-prevalence from ELISA and LAT, at cut-off of S/P 15.5% and ≥1:64, respectively, was not considerably totally different to that from WB (McNemar’s Chi-square p=0.21 for ELISA and p=0.28 for LAT).

Diagnostic efficiency of ELISA, IFAT and Western blot for the detection of anti-Leishmania infantum antibodies in cats utilizing a Bayesian evaluation with no gold commonplace.

Anti-Leishmania antibodies are more and more investigated in cats for epidemiological research or for the prognosis of medical feline leishmaniosis. The immunofluorescent antibody take a look at (IFAT), the enzyme-linked immunosorbent assay (ELISA) and western blot (WB) are the serological assessments extra continuously used. The intention of the current examine was to evaluate diagnostic efficiency of IFAT, ELISA and WB to detect anti-L. infantum antibodies in feline serum samples obtained from endemic (n = 76) and non-endemic (n = 64) areas and from cats affected by feline leishmaniosis (n = 21) by a Bayesian method with no gold commonplace.

METHODS

Lower-offs have been set at 80 titre for IFAT and 40 ELISA models for ELISA. WB was thought of constructive in presence of at the very least a 18 KDa band. Statistical evaluation was carried out by means of a written routine with MATLAB software program within the Bayesian framework. The latent knowledge and observations from the joint posterior have been simulated within the Bayesian method by an iterative Markov Chain Monte Carlo approach utilizing the Gibbs sampler for estimating sensitivity and specificity of the three assessments.

RESULTS

The median seroprevalence within the pattern used for evaluating the efficiency of assessments was estimated at 0.27 [credible interval (CI) = 0.20-0.34]. The median sensitivity of the three totally different strategies was 0.97 (CI: 0.86-1.00), 0.75 (CI: 0.61-0.87) and 0.70 (CI: 0.56-0.83) for WB, IFAT and ELISA, respectively.

Median specificity reached 0.99 (CI: 0.96-1.00) with WB, 0.97 (CI: 0.93-0.99) with IFAT and 0.98 (CI: 0.94-1.00) with ELISA. IFAT was extra delicate than ELISA (75 vs 70%) for the detection of subclinical an infection whereas ELISA was higher for diagnosing medical leishmaniosis compared with IFAT (98 vs 97%).

CONCLUSIONS

The general efficiency of all serological methods was good and probably the most correct take a look at for anti-Leishmania antibody detection in feline serum samples was WB.

EED Western Blot kit (AWBK38384)

AWBK38384 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

SSB Western Blot kit (AWBK40462)

AWBK40462 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

OTC Western Blot kit (AWBK41766)

AWBK41766 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

FST Western Blot kit (AWBK42287)

AWBK42287 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

TMB Substrate, for Western Blot

F100029 1000 ml
EUR 992.4
Description: TMB Substrate, for Western Blot by Cygnus Technologies is available in Europe via Gentaur.

TMB Substrate, for Western Blot

F129-100 100 ml
EUR 298.8
Description: TMB Substrate, for Western Blot by Cygnus Technologies is available in Europe via Gentaur.

ERG Western Blot kit (AWBK100625)

AWBK100625 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
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GLI2 Western Blot kit (AWBK31885)

AWBK31885 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
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PAX4 Western Blot kit (AWBK32064)

AWBK32064 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
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GLI1 Western Blot kit (AWBK32368)

AWBK32368 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
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PAX7 Western Blot kit (AWBK32393)

AWBK32393 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
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LHX6 Western Blot kit (AWBK32553)

AWBK32553 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
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PAX7 Western Blot kit (AWBK32742)

AWBK32742 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
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SOX5 Western Blot kit (AWBK33323)

AWBK33323 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

A1BG Western Blot kit (AWBK33810)

AWBK33810 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity: Human
  • Application:
  • Assay info:
  • Sensitivity:

NPM1 Western Blot kit (AWBK34094)

AWBK34094 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
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LIG4 Western Blot kit (AWBK34122)

AWBK34122 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
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RNF2 Western Blot kit (AWBK34290)

AWBK34290 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
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ILF2 Western Blot kit (AWBK35731)

AWBK35731 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
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SPP1 Western Blot kit (AWBK36677)

AWBK36677 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
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ZBP1 Western Blot kit (AWBK36711)

AWBK36711 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
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G3BP Western Blot kit (AWBK37713)

AWBK37713 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
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BRD7 Western Blot kit (AWBK39018)

AWBK39018 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
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MSI2 Western Blot kit (AWBK41112)

AWBK41112 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
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TPM1 Western Blot kit (AWBK41392)

AWBK41392 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
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TSHR Western Blot kit (AWBK41870)

AWBK41870 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
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ASPN Western Blot kit (AWBK42487)

AWBK42487 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
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RORA Western Blot kit (AWBK45608)

AWBK45608 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
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PDCD4 Western Blot kit (AWBK09045)

AWBK09045 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
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ETS1 Western Blot kit (AWBK100604)

AWBK100604 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
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GABRP Western Blot kit (AWBK13034)

AWBK13034 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
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HTR1A Western Blot kit (AWBK13041)

AWBK13041 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
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RAB14 Western Blot kit (AWBK13107)

AWBK13107 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
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TNRC4 Western Blot kit (AWBK31525)

AWBK31525 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
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ESRRG Western Blot kit (AWBK31655)

AWBK31655 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
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HIPK2 Western Blot kit (AWBK32586)

AWBK32586 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
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ZFP67 Western Blot kit (AWBK32749)

AWBK32749 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
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OLIG2 Western Blot kit (AWBK32753)

AWBK32753 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
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SOX10 Western Blot kit (AWBK33326)

AWBK33326 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
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RPS16 Western Blot kit (AWBK33535)

AWBK33535 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
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ATP7A Western Blot kit (AWBK33797)

AWBK33797 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

ZFP36 Western Blot kit (AWBK34385)

AWBK34385 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
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ARIH2 Western Blot kit (AWBK34418)

AWBK34418 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
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KEAP1 Western Blot kit (AWBK34728)

AWBK34728 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

CLIC1 Western Blot kit (AWBK35045)

AWBK35045 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

KCNN2 Western Blot kit (AWBK35094)

AWBK35094 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

CLCN3 Western Blot kit (AWBK35504)

AWBK35504 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

P2RX7 Western Blot kit (AWBK35518)

AWBK35518 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

TRPM3 Western Blot kit (AWBK35609)

AWBK35609 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

PITX2 Western Blot kit (AWBK35634)

AWBK35634 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

ZFP42 Western Blot kit (AWBK36244)

AWBK36244 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

ANXA1 Western Blot kit (AWBK36568)

AWBK36568 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

RUNX2 Western Blot kit (AWBK36678)

AWBK36678 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

Bapx1 Western Blot kit (AWBK36826)

AWBK36826 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

SATB1 Western Blot kit (AWBK38208)

AWBK38208 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

JMJD3 Western Blot kit (AWBK40102)

AWBK40102 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

STAU1 Western Blot kit (AWBK40537)

AWBK40537 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

PCBP1 Western Blot kit (AWBK40630)

AWBK40630 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

WNT2B Western Blot kit (AWBK41254)

AWBK41254 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

GNB1L Western Blot kit (AWBK41328)

AWBK41328 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

STK11 Western Blot kit (AWBK41743)

AWBK41743 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

KRT13 Western Blot kit (AWBK42031)

AWBK42031 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

IDH3A Western Blot kit (AWBK42237)

AWBK42237 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

PBEF1 Western Blot kit (AWBK42255)

AWBK42255 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

RSAD2 Western Blot kit (AWBK42696)

AWBK42696 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

PANX2 Western Blot kit (AWBK42778)

AWBK42778 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

PANX1 Western Blot kit (AWBK42783)

AWBK42783 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

TGFBI Western Blot kit (AWBK44268)

AWBK44268 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

PTPN2 Western Blot kit (AWBK45362)

AWBK45362 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

TWIST1 Western Blot kit (AWBK37997)

AWBK37997 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

TARDBP Western Blot kit (AWBK38941)

AWBK38941 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

TARDBP Western Blot kit (AWBK38942)

AWBK38942 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

SNRP70 Western Blot kit (AWBK40439)

AWBK40439 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

HNRPLL Western Blot kit (AWBK41102)

AWBK41102 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

HNRPA3 Western Blot kit (AWBK41195)

AWBK41195 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

SLC1A5 Western Blot kit (AWBK42247)

AWBK42247 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

ONECUT2 Western Blot kit (AWBK35750)

AWBK35750 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

SLC12A1 Western Blot kit (AWBK41388)

AWBK41388 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

CEACAM6 Western Blot kit (AWBK41504)

AWBK41504 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

SLC22A1 Western Blot kit (AWBK41516)

AWBK41516 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

RHOBTB1 Western Blot kit (AWBK41883)

AWBK41883 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

SLC22A7 Western Blot kit (AWBK42306)

AWBK42306 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
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Interferon alpha Western Blot Antibody

20-abx137014
  • EUR 427.20
  • EUR 627.60
  • 0.5 mg
  • 1 mg

Colorimetric Western Blot Kit (OKRA00014)

OKRA00014 1 kit
EUR 730.8
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

Colorimetric Western Blot Kit (OKRA00015)

OKRA00015 1 each
EUR 730.8
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

Colorimetric Western Blot Kit (OKRA00016)

OKRA00016 1 kit
EUR 730.8
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

Colorimetric Western Blot Kit (OKRA00017)

OKRA00017 1 kit
EUR 730.8
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

cyclin G2 Western Blot kit (AWBK03032)

AWBK03032 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

ChonBlock Western Blot Buffer 1 (PBS)

9069P 10 ml
EUR 232.26
Description: ChonBlock Western Blot Buffer 1 (PBS)

ChonBlock Western Blot Buffer 1 (TBS)

9069T 10 ml
EUR 232.26
Description: ChonBlock Western Blot Buffer 1 (TBS)

Pure-IP Western Blot Detection Kit

PRB-5002 20 blots
EUR 470.4
Description: Western blot detection of IP proteins can exhibit high background due to the use of the same primary antibody in the IP and the Western blot. The Pure-IP Western Blot Detection Kit eliminates this background problem by using a proprietary HRP Conjugate for detection of the primary antibody.

ChonBlock Western Blot Buffer 2 for Goat (PBS)

90691P 10 ml
EUR 145.26
Description: ChonBlock Western Blot Buffer 2 for Goat (PBS)

ChonBlock Western Blot Buffer 2 for Goat (TBS)

90691T 10 ml
EUR 145.26
Description: ChonBlock Western Blot Buffer 2 for Goat (TBS)

Chemiluminescent Western Blot Kit (OKRA00001)

OKRA00001 1 kit
EUR 684
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

Chemiluminescent Western Blot Kit (OKRA00002)

OKRA00002 1 kit
EUR 684
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

Chemiluminescent Western Blot Kit (OKRA00003)

OKRA00003 1 kit
EUR 684
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

Chemiluminescent Western Blot Kit (OKRA00004)

OKRA00004 1 kit
EUR 684
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

T-Pro Western Blot Stripping Reagent

JB11-K002 500ml/BT
EUR 213.6

ChonBlock Western Blot Buffer 2 for Rabbit (PBS)

90692P 10 ml
EUR 145.26
Description: ChonBlock Western Blot Buffer 2 for Rabbit (PBS)

ChonBlock Western Blot Buffer 2 for Rabbit (TBS)

90692T 10 ml
EUR 145.26
Description: ChonBlock Western Blot Buffer 2 for Rabbit (TBS)

Human Leptin protein Western blot +ve control # 2

LEP12-C 100 ul
EUR 343.2

AgileBlotter? Automated Western Blot Processor

ACT-ABH-40 each
EUR 20326.8

Nitrated Mol Wt Std for Western blot

NITT12-C 100 ul
EUR 343.2

Western Blot Primary Antibody Diluent Buffer

abx090689-100ml 100 ml
EUR 218.4

Attoglow Western Blot Analysis Kit:Blocking Agent

K3171250-3 40 g
EUR 68

Hypersensitive Western Blot Chemiluminescent Substrate

CR0001-10 20mL (sufficient reagents for 4000 cm2 of membrane)
EUR 200.4

Hypersensitive Western Blot Chemiluminescent Substrate

CR0001-25 50mL (sufficient reagents for 10000 cm2 of membrane)
EUR 376.8

Hypersensitive Western Blot Chemiluminescent Substrate

CR0001-5 10mL (sufficient reagents for 2000 cm2 of membrane)
EUR 139.2

Recombinant Human MutY Protein for Western blot

MUTY11-C 100 ul
EUR 343.2

Purified Ram Cyclooxygenase (Cox-2) Western blot +ve control

COX21-C 100 ul
EUR 343.2

West-Ez Blocking Buffer, 1 % Casein for Western Blot

W3720-050 500ml
EUR 144

West-Ez Blocking Buffer, 1 % Casein for Western Blot

W3720-100 2x500ml
EUR 176.4

Poly-ADP-ribosylated proteins for Western blot

ADPR11-C 100 ul
EUR 343.2

Rat Ceruloplasmin protein control for western blot

CP17-C 100 ul
EUR 343.2

Attoglow Western Blot Analysis Kit:Binding Buffer 20x

K3171250-2 120 ml
EUR 107

SuperBrite? ECL Western Blot Substrate/Detection Kit

K824-50 each
EUR 229.2