March 19, 2024

Evaluation in animal fashions of myocardial ischemic infarction

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An acceptable loading management for western blot evaluation in animal fashions of myocardial ischemic infarction.

An acceptable loading management is crucial for Western blot evaluation. Housekeeping proteins (HKPs), corresponding to β-actin, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and β-tubulin, are generally used to normalize protein expression.

However HKP expression will be impacted by sure experimental situations, corresponding to ischemic myocardial infarction. This examine was undertaken to search for an acceptable loading management for western blot evaluation of ischemic myocardium.

Myocardial ischemic infarction was induced by left anterior descending coronary artery (LAD) ligation in Rhesus monkeys and C57BL/6 mice. The guts tissue samples from totally different areas and time factors after surgical procedure have been subjected to western blot or gel staining.

The extent of β-actin, GAPDH, β-tubulin, and whole protein have been examined. The full protein stage was constant in all teams, whereas the protein stage of β-tubulin and β-actin have been totally different in all teams.

Nonetheless, the protein stage of GAPDH was secure within the Rhesus monkey mannequin. We concluded that whole protein was probably the most acceptable inside management in numerous phases of myocardial ischemic illness of assorted animal fashions. GAPDH is a dependable inside management just for ischemic myocardium of Rhesus monkey.

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Chicken Tissue Custom Made Western Blot-1

CW-CUS-1 1 Blot
EUR 668

Pig Tissue Custom Made Western Blot-2

PW-CUS-2 1 Blot
EUR 789

Dog Tissue Custom Made Western Blot-2

DW-CUS-2 1 Blot
EUR 910

Sheep Tissue Custom Made Western Blot-2

SW-CUS-2 1 Blot
EUR 789

Human Tissue Custom Made Western Blot-2

HW-CUS-2 1 Blot
EUR 1031

Plant Tissue Custom Made Western Blot-2

LW-CUS-2 1 Blot
EUR 1086

Rabbit Tissue Custom Made Western Blot-2

TW-CUS-2 1 Blot
EUR 789

Bovine Tissue Custom Made Western Blot-2

BW-CUS-2 1 Blot
EUR 789

Equine Tissue Custom Made Western Blot-2

EW-CUS-2 1 Blot
EUR 910

Minipig Tissue Custom Made Western Blot-2

NW-CUS-2 1 Blot
EUR 910

Hamster Tissue Custom Made Western Blot-2

AW-CUS-2 1 Blot
EUR 789

Chicken Tissue Custom Made Western Blot-2

CW-CUS-2 1 Blot
EUR 789

Guinea Pig Tissue Custom Made Western Blot-1

GW-CUS-1 1 Blot
EUR 668

Monkey Cyno Tissue Custom Made Western Blot-1

KW-CUS-1 1 Blot
EUR 789

Monkey Rhesus Tissue Custom Made Western Blot-1

UW-CUS-1 1 Blot
EUR 789

Panel 7: Rat Tissue Custom Made Western Blot-1

RW-CUS-1 1 Blot
EUR 722

Guinea Pig Tissue Custom Made Western Blot-2

GW-CUS-2 1 Blot
EUR 789

Monkey Cyno Tissue Custom Made Western Blot-2

KW-CUS-2 1 Blot
EUR 910

Monkey Rhesus Tissue Custom Made Western Blot-2

UW-CUS-2 1 Blot
EUR 910

Panel 8: Rat Tissue Custom Made Western Blot-2

RW-CUS-2 1 Blot
EUR 843

Panel 9: Rat Tissue Custom Made Western Blot-3

RW-CUS-3 1 Blot
EUR 965

Panel 7: Mouse CD1 Tissue Custom Made Western Blot-1

MW-CUS-1 1 Blot
EUR 722

Panel 7: Mouse C57 Tissue Custom Made Western Blot-1

MW-CUS-1-C57 1 Blot
EUR 843

Panel 8: Mouse CD1 Tissue Custom Made Western Blot-2

MW-CUS-2 1 Blot
EUR 843

Panel 8: Mouse C57 Tissue Custom Made Western Blot-2

MW-CUS-2-C57 1 Blot
EUR 965

Panel 9: Mouse CD1 Tissue Custom Made Western Blot-3

MW-CUS-3 1 Blot
EUR 965

Panel 9: Mouse C57 Tissue Custom Made Western Blot-3

MW-CUS-3-C57 1 Blot
EUR 1086

Dog Tissue Custom Made Northern Blot-1

DN-CUS-1 1 Blot
EUR 910

Plant Tissue Custom Made Northern Blot-1

LN-CUS-1 1 Blot
EUR 910

Dog Tissue Custom Made Northern Blot-2

DN-CUS-2 1 Blot
EUR 1031

Plant Tissue Custom Made Northern Blot-2

LN-CUS-2 1 Blot
EUR 1153

Panel 7: Rat Tissue Custom Made Northern Blot-1

RN-CUS-1 1 Blot
EUR 722

Panel 8: Rat Tissue Custom Made Northern Blot-2

RN-CUS-2 1 Blot
EUR 843

Panel 9: Rat Tissue Custom Made Northern Blot-3

RN-CUS-3 1 Blot
EUR 965

Panel 7: Mouse CD1 Tissue Custom Made Northern Blot-1

MN-CUS-1 1 Blot
EUR 722

Panel 7: Mouse C57 Tissue Custom Made Northern Blot-1

MN-CUS-1-C57 1 Blot
EUR 910

Panel 8: Mouse CD1 Tissue Custom Made Northern Blot-2

MN-CUS-2 1 Blot
EUR 843

Panel 8: Mouse C57 Tissue Custom Made Northern Blot-2

MN-CUS-2-C57 1 Blot
EUR 1031

Panel 9: Mouse CD1 Tissue Custom Made Northern Blot-3

MN-CUS-3 1 Blot
EUR 965

Panel 9: Mouse C57 Tissue Custom Made Northern Blot-3

MN-CUS-3-C57 1 Blot
EUR 1153

Pig Tissue Custom Made NB-1

PN-CUS-1 1 Blot
EUR 789

Sheep Tissue Custom Made NB-1

SN-CUS-1 1 Blot
EUR 789

Human Tissue Custom Made NB-1

HN-CUS-1 1 Blot
EUR 910

Rabbit Tissue Custom Made NB-1

TN-CUS-1 1 blot
EUR 789

Bovine Tissue Custom Made NB-1

BN-CUS-1 1 Blot
EUR 789

Equine Tissue Custom Made NB-1

EN-CUS-1 1 Blot
EUR 910

Minipig Tissue Custom Made NB-1

NN-CUS-1 1 Blot
EUR 910

Hamster Tissue Custom Made NB-1

AN-CUS-1 1 Blot
EUR 789

Chicken Tissue Custom Made NB-1

CN-CUS-1 1 blot
EUR 789

Pig Tissue Custom Made NB-2

PN-CUS-2 1 Blot
EUR 910

Guinea Pig Tissue Custom Made NB-1

GN-CUS-1 1 Blot
EUR 789

Sheep Tissue Custom Made NB-2

SN-CUS-2 1 Blot
EUR 910

Human Tissue Custom Made NB-2

HN-CUS-2 1 Blot
EUR 1031

Monkey Cyno Tissue Custom Made NB-1

KN-CUS-1 1 Blot
EUR 910

Rabbit Tissue Custom Made NB-2

TN-CUS-2 1 Blot
EUR 910

Bovine Tissue Custom Made NB-2

BN-CUS-2 1 Blot
EUR 910

Equine Tissue Custom Made NB-2

EN-CUS-2 1 Blot
EUR 1031

Minipig Tissue Custom Made NB-2

NN-CUS-2 1 Blot
EUR 1031

Hamster Tissue Custom Made NB-2

AN-CUS-2 1 Blot
EUR 910

Chicken Tissue Custom Made NB-2

CN-CUS-2 1 blot
EUR 910

Monkey Rhesus Tissue Custom Made NB-1

UN-CUS-1 1 Blot
EUR 910

Guinea Pig Tissue Custom Made NB-2

GN-CUS-2 1 Blot
EUR 910

Monkey Cyno Tissue Custom Made NB-2

KN-CUS-2 1 Blot
EUR 1031

Monkey Rhesus Tissue Custom Made NB-2

UN-CUS-2 1 Blot
EUR 1031

Pig Tissue Premade Western Blot

PW-MT-1 1 Blot
EUR 910

Pig Tissue Premade Western Blot

PW-MT-1-1 0.1mg
EUR 910

Dog Tissue Premade Western Blot

DW-MT-1 1 Blot
EUR 1031

Sheep Tissue Premade Western Blot

SW-MT-1 1 Blot
EUR 910

Human Tissue Premade Western Blot

HW-MT-1 1 Blot
EUR 1153

Plant Tissue Premade Western Blot

LW-MT-1 1 Blot
EUR 1208

Rabbit Tissue Premade Western Blot

TW-MT-1 1 Blot
EUR 910

Bovine Tissue Premade Western Blot

BW-MT-1 1 Blot
EUR 910

Equine Tissue Premade Western Blot

EW-MT-1 1 Blot
EUR 1031

Minipig Tissue Premade Western Blot

NW-MT-1 1 Blot
EUR 1031

Hamster Tissue Premade Western Blot

AW-MT-1 1 Blot
EUR 910

Chicken Tissue Premade Western Blot

CW-MT-1 1 Blot
EUR 910

Guinea Pig Tissue Premade Western Blot

GW-MT-1 1 Blot
EUR 910

Monkey Cyo Tissue Premade Western Blot

KW-MT-1 1 Blot
EUR 1031

Monkey Cyo Tissue Premade Western Blot

KW-MT-1-1 1 Blot
EUR 1031

Monkey Rhesus Tissue Premade Western Blot

UW-MT-1 1 Blot
EUR 1031

Panel 1: Rat Major Tissue Premade Western Blot

RW-MT-1 1 Blot
EUR 965

Panel 1: Mouse CD1 Major Tissue Premade Western Blot

MW-MT-1 1 Blot
EUR 965

Panel 1: Mouse C57 Major Tissue Premade Western Blot

MW-MT-1-C57 1 Blot
EUR 1086

Panel 2: Rat Minor Tissue Premade Western Blot

RW-MT-2 1 Blot
EUR 843

Panel 3: Rat Neuronal Tissue Premade Western Blot

RW-MT-3 1 Blot
EUR 601

Panel 4: Rat Digestive Tissue Premade Western Blot

RW-MT-4 1 Blot
EUR 601

Panel 5: Rat Reproductive Tissue Premade Western Blot

RW-MT-5 1 Blot
EUR 601

Panel 2: Mouse CD1 Minor Tissue Premade Western Blot

MW-MT-2 1 Blot
EUR 843

Panel 2: Mouse C57 Minor Tissue Premade Western Blot

MW-MT-2-C57 1 Blot
EUR 965

Panel 3: Mouse CD1 Neuronal Tissue Premade Western Blot

MW-MT-3 1 Blot
EUR 601

Panel 3: Mouse C57 Neuronal Tissue Premade Western Blot

MW-MT-3-C57 1 Blot
EUR 722

OPRB00269-10UG - Tissue Factor (Western Blot Control) Protein

OPRB00269-10UG 10ug
EUR 256

Panel 4: Mouse CD1 Digestive Tissue Premade Western Blot

MW-MT-4 1 Blot
EUR 601

Panel 4: Mouse C57 Digestive Tissue Premade Western Blot

MW-MT-4-C57 1 Blot
EUR 722

Panel 5: Mouse CD1 Reproductive Tissue Premade Western Blot

MW-MT-5 1 Blot
EUR 601

Panel 5: Mouse C57 Reproductive Tissue Premade Western Blot

MW-MT-5-C57 1 Blot
EUR 722

Panel 6: Rat Cir, Res, Uri, Imm Tissue Premade Western Blot

RW-MT-6 1 Blot
EUR 601

Panel 6: Mouse CD1 Cir, Res, Uri, Imm Tissue Premade Western Blot

MW-MT-6 1 Blot
EUR 601

Panel 6: Mouse C57 Cir, Res, Uri, Imm Tissue Premade Western Blot

MW-MT-6-C57 1 Blot
EUR 722

Total Protein Western Blots - Human Adult Normal Tissue, Blot IV, 16 lanes

W1234404 1
EUR 995

OPRB00085-10UG - Tissue Inhibitor of Metalloproteinase 1 (Western Blot Cotrol) Protein

OPRB00085-10UG 10ug
EUR 256

Western Blot Kit

20-abx098123
  • EUR 276.00
  • EUR 343.20
  • 100 ml
  • 200 ml

Western Blot Blocker

20960007-1 250 g
EUR 32.46

Western Blot Blocker

20960007-2 500 g
EUR 60.54

Western Blot Marker

TTOPP-2301 20Lanes
EUR 310.44

Rat Placenta Western Blot

RW-413 1 Blot
EUR 668

Western Blot Enhancing Kit

K3172120 1200 cm2
EUR 107

Western Blot Enhancing Kit

K3172250 2500 cm2
EUR 158

ChonBlock Western Blot Buffer 1 (PBS)

9069P 10 ml
EUR 232.26
Description: ChonBlock Western Blot Buffer 1 (PBS)

ChonBlock Western Blot Buffer 1 (TBS)

9069T 10 ml
EUR 232.26
Description: ChonBlock Western Blot Buffer 1 (TBS)

Skin Western Blot, 14 species

AW-101 1 Blot
EUR 1031

Lung Western Blot, 14 species

AW-601 1 Blot
EUR 1031

Rat Whole Embryo Western Blot

RW-104 1 Blot
EUR 668

Brain Western Blot, 14 species

AW-201 1 Blot
EUR 1031

Colon Western Blot, 14 species

AW-311 1 Blot
EUR 1031

Liver Western Blot, 14 species

AW-314 1 Blot
EUR 1031

Ovary Western Blot, 14 species

AW-406 1 Blot
EUR 1031

Heart Western Blot, 14 species

AW-801 1 Blot
EUR 1031

AHR Western Blot kit (AWBK32243)

AWBK32243 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

ENG Western Blot kit (AWBK33068)

AWBK33068 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

ID4 Western Blot kit (AWBK38058)

AWBK38058 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

EED Western Blot kit (AWBK38384)

AWBK38384 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

SSB Western Blot kit (AWBK40462)

AWBK40462 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

OTC Western Blot kit (AWBK41766)

AWBK41766 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

FST Western Blot kit (AWBK42287)

AWBK42287 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

TMB Substrate, for Western Blot

F100029 1000 ml
EUR 992.4
Description: TMB Substrate, for Western Blot by Cygnus Technologies is available in Europe via Gentaur.

TMB Substrate, for Western Blot

F129-100 100 ml
EUR 298.8
Description: TMB Substrate, for Western Blot by Cygnus Technologies is available in Europe via Gentaur.

Rat Mammary Gland Western Blot

RW-414 1 Blot
EUR 668

Testis Western Blot, 14 species

AW-401 1 Blot
EUR 1031

Kidney Western Blot, 14 species

AW-901 1 Blot
EUR 1031

ERG Western Blot kit (AWBK100625)

AWBK100625 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

GLI2 Western Blot kit (AWBK31885)

AWBK31885 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

PAX4 Western Blot kit (AWBK32064)

AWBK32064 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

GLI1 Western Blot kit (AWBK32368)

AWBK32368 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

PAX7 Western Blot kit (AWBK32393)

AWBK32393 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

LHX6 Western Blot kit (AWBK32553)

AWBK32553 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

PAX7 Western Blot kit (AWBK32742)

AWBK32742 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

SOX5 Western Blot kit (AWBK33323)

AWBK33323 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

A1BG Western Blot kit (AWBK33810)

AWBK33810 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity: Human
  • Application:
  • Assay info:
  • Sensitivity:

NPM1 Western Blot kit (AWBK34094)

AWBK34094 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

LIG4 Western Blot kit (AWBK34122)

AWBK34122 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

RNF2 Western Blot kit (AWBK34290)

AWBK34290 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

ILF2 Western Blot kit (AWBK35731)

AWBK35731 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

SPP1 Western Blot kit (AWBK36677)

AWBK36677 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

ZBP1 Western Blot kit (AWBK36711)

AWBK36711 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

G3BP Western Blot kit (AWBK37713)

AWBK37713 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

BRD7 Western Blot kit (AWBK39018)

AWBK39018 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

MSI2 Western Blot kit (AWBK41112)

AWBK41112 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

TPM1 Western Blot kit (AWBK41392)

AWBK41392 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

TSHR Western Blot kit (AWBK41870)

AWBK41870 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

ASPN Western Blot kit (AWBK42487)

AWBK42487 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

RORA Western Blot kit (AWBK45608)

AWBK45608 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

Mouse CD1 Placenta Western Blot

MW-413 1 Blot
EUR 668

Mouse C57 Placenta Western Blot

MW-413-C57 1 Blot
EUR 789

Analysis of Western blot, ELISA and latex agglutination assessments to detect Toxoplasma gondii serum antibodies in farmed purple deer.

Abortion as a result of Toxoplasma gondii has been suspected in New Zealand farmed purple deer. Nonetheless, data across the epidemiology and prevalence of T. gondii in farmed purple deer is proscribed. The intention of this examine was to firstly, assess the sensitivity and specificity of two commercially accessible assays, ELISA and latex agglutination take a look at (LAT), to be used in deer and secondly, to estimate the sero-prevalence of T. gondii in purple deer. A complete of 252 sera from rising 2-year-old and grownup hinds from 17 New Zealand purple deer herds at early and late being pregnant scanning and from recognized aborted and/or non-aborted hinds have been examined for the presence of T. gondii antibodies.

Every assays’ sensitivity and specificity was evaluated by each the Western Blot (WB) as a gold commonplace technique and Bayesian latent class (BLC) evaluation within the absence of a gold commonplace.

The sensitivity and specificity for WB have been 95.8% (95% credible interval: 89.5-99.2%) and 95.1% (95% credible interval: 90.6-98.1%), respectively. For the LAT on the producer’s advisable ≥1:32 cut-off titre, the sensitivity (88.7%, 95% credible interval: 80.8-94.7%) and specificity (74.3%, 95% credible interval: 67.5-80.5%) have been decrease and better than the sensitivity (76.2%, 95% credible interval: 66.7-84.5%) and specificity (89.7%, 95% credible interval: 84.5-93.9%) at a ≥1:64 cut-off, utilizing (BLC) evaluation.

Sensitivity and specificity of the LAT at cut-off titre of 1:32 have been estimated to be 84.4% (95% CI: 74.9-90.9%) and 73.5% (95% CI: 65.8-79.9%) towards WB. The LAT had higher settlement with WB at cut-off titre of ≥1:64 than ≥1:32 (Kappa=0.63 vs 0.54). At optimised cut-off S/P of 15.5%, the sensitivity (98.8%, 95% credible interval 96.1-99.8%) and specificity (92.8%, 95% credible interval 88.9-95.7%) of the ELISA have been greater and decrease, respectively, than the sensitivity (85.1%, 95% credible interval 76.2-91.9%) and specificity (98.5%, 95% credible interval 96.9-99.4%) at producer’s cut-off S/P of 30%, from BLC evaluation. The sensitivity and specificity of ELISA at S/P cut-off of 15.5% was 91.1% (95% CI: 83.2-96.1%) and 90.7% (95% CI: 85.2-94.7%), respectively, when assessed towards WB. The sero-prevalence from ELISA and LAT, at cut-off of S/P 15.5% and ≥1:64, respectively, was not considerably totally different to that from WB (McNemar’s Chi-square p=0.21 for ELISA and p=0.28 for LAT).

Diagnostic efficiency of ELISA, IFAT and Western blot for the detection of anti-Leishmania infantum antibodies in cats utilizing a Bayesian evaluation with no gold commonplace.

Anti-Leishmania antibodies are more and more investigated in cats for epidemiological research or for the prognosis of medical feline leishmaniosis. The immunofluorescent antibody take a look at (IFAT), the enzyme-linked immunosorbent assay (ELISA) and western blot (WB) are the serological assessments extra continuously used. The intention of the current examine was to evaluate diagnostic efficiency of IFAT, ELISA and WB to detect anti-L. infantum antibodies in feline serum samples obtained from endemic (n = 76) and non-endemic (n = 64) areas and from cats affected by feline leishmaniosis (n = 21) by a Bayesian method with no gold commonplace.

METHODS

Lower-offs have been set at 80 titre for IFAT and 40 ELISA models for ELISA. WB was thought of constructive in presence of at the very least a 18 KDa band. Statistical evaluation was carried out by means of a written routine with MATLAB software program within the Bayesian framework. The latent knowledge and observations from the joint posterior have been simulated within the Bayesian method by an iterative Markov Chain Monte Carlo approach utilizing the Gibbs sampler for estimating sensitivity and specificity of the three assessments.

RESULTS

The median seroprevalence within the pattern used for evaluating the efficiency of assessments was estimated at 0.27 [credible interval (CI) = 0.20-0.34]. The median sensitivity of the three totally different strategies was 0.97 (CI: 0.86-1.00), 0.75 (CI: 0.61-0.87) and 0.70 (CI: 0.56-0.83) for WB, IFAT and ELISA, respectively.

Median specificity reached 0.99 (CI: 0.96-1.00) with WB, 0.97 (CI: 0.93-0.99) with IFAT and 0.98 (CI: 0.94-1.00) with ELISA. IFAT was extra delicate than ELISA (75 vs 70%) for the detection of subclinical an infection whereas ELISA was higher for diagnosing medical leishmaniosis compared with IFAT (98 vs 97%).

CONCLUSIONS

The general efficiency of all serological methods was good and probably the most correct take a look at for anti-Leishmania antibody detection in feline serum samples was WB.

Equine Tissue Custom Made Western Blot-2

EW-CUS-2 1 Blot
EUR 910

Minipig Tissue Custom Made Western Blot-2

NW-CUS-2 1 Blot
EUR 910

Hamster Tissue Custom Made Western Blot-2

AW-CUS-2 1 Blot
EUR 789

Chicken Tissue Custom Made Western Blot-2

CW-CUS-2 1 Blot
EUR 789

Pig Tissue Custom Made Western Blot-1

PW-CUS-1 1 Blot
EUR 668

Sheep Tissue Custom Made Western Blot-1

SW-CUS-1 1 Blot
EUR 668

Human Tissue Custom Made Western Blot-1

HW-CUS-1 1 Blot
EUR 910

Plant Tissue Custom Made Western Blot-1

LW-CUS-1 1 Blot
EUR 843

Rabbit Tissue Custom Made Western Blot-1

TW-CUS-1 1 Blot
EUR 668

Bovine Tissue Custom Made Western Blot-1

BW-CUS-1 1 Blot
EUR 668

Equine Tissue Custom Made Western Blot-1

EW-CUS-1 1 Blot
EUR 789

Guinea Pig Tissue Custom Made Western Blot-2

GW-CUS-2 1 Blot
EUR 789

Minipig Tissue Custom Made Western Blot-1

NW-CUS-1 1 Blot
EUR 789

Hamster Tissue Custom Made Western Blot-1

AW-CUS-1 1 Blot
EUR 668

Chicken Tissue Custom Made Western Blot-1

CW-CUS-1 1 Blot
EUR 668

Monkey Cyno Tissue Custom Made Western Blot-2

KW-CUS-2 1 Blot
EUR 910

Monkey Rhesus Tissue Custom Made Western Blot-2

UW-CUS-2 1 Blot
EUR 910

Panel 8: Rat Tissue Custom Made Western Blot-2

RW-CUS-2 1 Blot
EUR 843

Guinea Pig Tissue Custom Made Western Blot-1

GW-CUS-1 1 Blot
EUR 668

Monkey Cyno Tissue Custom Made Western Blot-1

KW-CUS-1 1 Blot
EUR 789

Monkey Rhesus Tissue Custom Made Western Blot-1

UW-CUS-1 1 Blot
EUR 789

Panel 7: Rat Tissue Custom Made Western Blot-1

RW-CUS-1 1 Blot
EUR 722

Panel 9: Rat Tissue Custom Made Western Blot-3

RW-CUS-3 1 Blot
EUR 965

Panel 8: Mouse CD1 Tissue Custom Made Western Blot-2

MW-CUS-2 1 Blot
EUR 843

Panel 8: Mouse C57 Tissue Custom Made Western Blot-2

MW-CUS-2-C57 1 Blot
EUR 965

Panel 7: Mouse CD1 Tissue Custom Made Western Blot-1

MW-CUS-1 1 Blot
EUR 722

Panel 7: Mouse C57 Tissue Custom Made Western Blot-1

MW-CUS-1-C57 1 Blot
EUR 843

Panel 9: Mouse CD1 Tissue Custom Made Western Blot-3

MW-CUS-3 1 Blot
EUR 965

Panel 9: Mouse C57 Tissue Custom Made Western Blot-3

MW-CUS-3-C57 1 Blot
EUR 1086

Dog Tissue Custom Made Northern Blot-2

DN-CUS-2 1 Blot
EUR 1031

Dog Tissue Custom Made Northern Blot-1

DN-CUS-1 1 Blot
EUR 910

Plant Tissue Custom Made Northern Blot-2

LN-CUS-2 1 Blot
EUR 1153

Plant Tissue Custom Made Northern Blot-1

LN-CUS-1 1 Blot
EUR 910

Panel 8: Rat Tissue Custom Made Northern Blot-2

RN-CUS-2 1 Blot
EUR 843

Panel 8: Mouse CD1 Tissue Custom Made Northern Blot-2

MN-CUS-2 1 Blot
EUR 843

Panel 8: Mouse C57 Tissue Custom Made Northern Blot-2

MN-CUS-2-C57 1 Blot
EUR 1031

Panel 7: Rat Tissue Custom Made Northern Blot-1

RN-CUS-1 1 Blot
EUR 722

Panel 9: Rat Tissue Custom Made Northern Blot-3

RN-CUS-3 1 Blot
EUR 965

Panel 7: Mouse CD1 Tissue Custom Made Northern Blot-1

MN-CUS-1 1 Blot
EUR 722

Panel 7: Mouse C57 Tissue Custom Made Northern Blot-1

MN-CUS-1-C57 1 Blot
EUR 910

Panel 9: Mouse CD1 Tissue Custom Made Northern Blot-3

MN-CUS-3 1 Blot
EUR 965

Panel 9: Mouse C57 Tissue Custom Made Northern Blot-3

MN-CUS-3-C57 1 Blot
EUR 1153

Pig Tissue Custom Made NB-2

PN-CUS-2 1 Blot
EUR 910

Sheep Tissue Custom Made NB-2

SN-CUS-2 1 Blot
EUR 910

Human Tissue Custom Made NB-2

HN-CUS-2 1 Blot
EUR 1031

Rabbit Tissue Custom Made NB-2

TN-CUS-2 1 Blot
EUR 910

Bovine Tissue Custom Made NB-2

BN-CUS-2 1 Blot
EUR 910

Equine Tissue Custom Made NB-2

EN-CUS-2 1 Blot
EUR 1031

Minipig Tissue Custom Made NB-2

NN-CUS-2 1 Blot
EUR 1031

Hamster Tissue Custom Made NB-2

AN-CUS-2 1 Blot
EUR 910

Chicken Tissue Custom Made NB-2

CN-CUS-2 1 blot
EUR 910

Guinea Pig Tissue Custom Made NB-2

GN-CUS-2 1 Blot
EUR 910

Pig Tissue Custom Made NB-1

PN-CUS-1 1 Blot
EUR 789

Monkey Cyno Tissue Custom Made NB-2

KN-CUS-2 1 Blot
EUR 1031

Sheep Tissue Custom Made NB-1

SN-CUS-1 1 Blot
EUR 789

Human Tissue Custom Made NB-1

HN-CUS-1 1 Blot
EUR 910

Monkey Rhesus Tissue Custom Made NB-2

UN-CUS-2 1 Blot
EUR 1031

Rabbit Tissue Custom Made NB-1

TN-CUS-1 1 blot
EUR 789

Bovine Tissue Custom Made NB-1

BN-CUS-1 1 Blot
EUR 789

Equine Tissue Custom Made NB-1

EN-CUS-1 1 Blot
EUR 910

Minipig Tissue Custom Made NB-1

NN-CUS-1 1 Blot
EUR 910

Hamster Tissue Custom Made NB-1

AN-CUS-1 1 Blot
EUR 789

Chicken Tissue Custom Made NB-1

CN-CUS-1 1 blot
EUR 789

Guinea Pig Tissue Custom Made NB-1

GN-CUS-1 1 Blot
EUR 789

Monkey Cyno Tissue Custom Made NB-1

KN-CUS-1 1 Blot
EUR 910

Monkey Rhesus Tissue Custom Made NB-1

UN-CUS-1 1 Blot
EUR 910

Dog Tissue Premade Western Blot

DW-MT-1 1 Blot
EUR 1031

Pig Tissue Premade Western Blot

PW-MT-1 1 Blot
EUR 910

Pig Tissue Premade Western Blot

PW-MT-1-1 0.1mg
EUR 910

Sheep Tissue Premade Western Blot

SW-MT-1 1 Blot
EUR 910

Human Tissue Premade Western Blot

HW-MT-1 1 Blot
EUR 1153

Plant Tissue Premade Western Blot

LW-MT-1 1 Blot
EUR 1208

Rabbit Tissue Premade Western Blot

TW-MT-1 1 Blot
EUR 910

Bovine Tissue Premade Western Blot

BW-MT-1 1 Blot
EUR 910

Equine Tissue Premade Western Blot

EW-MT-1 1 Blot
EUR 1031

Minipig Tissue Premade Western Blot

NW-MT-1 1 Blot
EUR 1031

Hamster Tissue Premade Western Blot

AW-MT-1 1 Blot
EUR 910

Chicken Tissue Premade Western Blot

CW-MT-1 1 Blot
EUR 910

Guinea Pig Tissue Premade Western Blot

GW-MT-1 1 Blot
EUR 910

Monkey Cyo Tissue Premade Western Blot

KW-MT-1 1 Blot
EUR 1031

Monkey Cyo Tissue Premade Western Blot

KW-MT-1-1 1 Blot
EUR 1031

Panel 2: Rat Minor Tissue Premade Western Blot

RW-MT-2 1 Blot
EUR 843

Monkey Rhesus Tissue Premade Western Blot

UW-MT-1 1 Blot
EUR 1031

Panel 2: Mouse CD1 Minor Tissue Premade Western Blot

MW-MT-2 1 Blot
EUR 843

Panel 2: Mouse C57 Minor Tissue Premade Western Blot

MW-MT-2-C57 1 Blot
EUR 965

Panel 1: Rat Major Tissue Premade Western Blot

RW-MT-1 1 Blot
EUR 965

Panel 3: Rat Neuronal Tissue Premade Western Blot

RW-MT-3 1 Blot
EUR 601

Panel 4: Rat Digestive Tissue Premade Western Blot

RW-MT-4 1 Blot
EUR 601

Panel 5: Rat Reproductive Tissue Premade Western Blot

RW-MT-5 1 Blot
EUR 601

Panel 1: Mouse CD1 Major Tissue Premade Western Blot

MW-MT-1 1 Blot
EUR 965

Panel 1: Mouse C57 Major Tissue Premade Western Blot

MW-MT-1-C57 1 Blot
EUR 1086

Panel 3: Mouse CD1 Neuronal Tissue Premade Western Blot

MW-MT-3 1 Blot
EUR 601

Panel 3: Mouse C57 Neuronal Tissue Premade Western Blot

MW-MT-3-C57 1 Blot
EUR 722

OPRB00269-10UG - Tissue Factor (Western Blot Control) Protein

OPRB00269-10UG 10ug
EUR 256

Panel 4: Mouse CD1 Digestive Tissue Premade Western Blot

MW-MT-4 1 Blot
EUR 601

Panel 4: Mouse C57 Digestive Tissue Premade Western Blot

MW-MT-4-C57 1 Blot
EUR 722

Panel 5: Mouse CD1 Reproductive Tissue Premade Western Blot

MW-MT-5 1 Blot
EUR 601

Panel 5: Mouse C57 Reproductive Tissue Premade Western Blot

MW-MT-5-C57 1 Blot
EUR 722

Panel 6: Rat Cir, Res, Uri, Imm Tissue Premade Western Blot

RW-MT-6 1 Blot
EUR 601

Panel 6: Mouse CD1 Cir, Res, Uri, Imm Tissue Premade Western Blot

MW-MT-6 1 Blot
EUR 601

Panel 6: Mouse C57 Cir, Res, Uri, Imm Tissue Premade Western Blot

MW-MT-6-C57 1 Blot
EUR 722

Total Protein Western Blots - Human Adult Normal Tissue, Blot IV, 16 lanes

W1234404 1
EUR 995

OPRB00085-10UG - Tissue Inhibitor of Metalloproteinase 1 (Western Blot Cotrol) Protein

OPRB00085-10UG 10ug
EUR 256

Western Blot Kit

20-abx098123
  • EUR 276.00
  • EUR 343.20
  • 100 ml
  • 200 ml

Western Blot Blocker

20960007-1 250 g
EUR 32.46

Western Blot Blocker

20960007-2 500 g
EUR 60.54

Western Blot Marker

TTOPP-2301 20Lanes
EUR 310.44

Dog Tissue Premade Northern Blot

DN-MT-1 1 Blot
EUR 1153

Rat Placenta Western Blot

RW-413 1 Blot
EUR 668

Western Blot Enhancing Kit

K3172120 1200 cm2
EUR 107

Western Blot Enhancing Kit

K3172250 2500 cm2
EUR 158

Skin Western Blot, 14 species

AW-101 1 Blot
EUR 1031

Lung Western Blot, 14 species

AW-601 1 Blot
EUR 1031

Rat Whole Embryo Western Blot

RW-104 1 Blot
EUR 668

Brain Western Blot, 14 species

AW-201 1 Blot
EUR 1031

Colon Western Blot, 14 species

AW-311 1 Blot
EUR 1031